Evaluation of an accelerated hydrogen peroxide disinfectant to inactivate porcine epidemic diarrhea virus in swine feces on aluminum surfaces under freezing conditions
Identifieur interne : 000789 ( Main/Exploration ); précédent : 000788; suivant : 000790Evaluation of an accelerated hydrogen peroxide disinfectant to inactivate porcine epidemic diarrhea virus in swine feces on aluminum surfaces under freezing conditions
Auteurs : Kimberlee L. Baker [États-Unis] ; Paul R. Thomas [États-Unis] ; Locke A. Karriker [États-Unis] ; Alejandro Ramirez [États-Unis] ; Jianqiang Zhang [États-Unis] ; Chong Wang [États-Unis] ; Derald J. Holtkamp [États-Unis]Source :
- BMC Veterinary Research [ 1746-6148 ] ; 2017.
Abstract
Since its emergence in 2013, porcine epidemic diarrhea virus (PEDV) spread rapidly throughout the country due, in part, to contaminated livestock trailers. The objective of this study was to test the efficacy of an accelerated hydrogen peroxide (AHP) disinfectant for inactivating PEDV in swine feces on metal surfaces under freezing conditions. One 15.24 X 15.24 X 2.54 cm aluminum coupon, contaminated with swine feces, and randomly matched to one pig was the experimental unit. Eight treatment groups representing two AHP concentrations (1:16 and 1:32) in a 10% propylene glycol solution, two contact times in a -10 °C freezer (40 min and 60 min), and two levels of fecal contamination (5 mL and 10 mL) in addition to negative and positive control groups were evaluated. Forty 3-week-old pigs, intragastrically inoculated with the contents of the coupons after treatment, were used as a bioassay to determine the infectivity of PEDV after treatment. Infectivity was determined by detection of virus with a nucleocapsid (N) gene-based quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR) on rectal swabs collected from the inoculated pigs on days three and seven post-inoculation.
All post-treatment swabs from the negative control coupons were negative for PEDV via RT-qPCR. All post-treatment swabs collected from coupons in the AHP disinfectant treatment groups and the positive control group were positive for PEDV via RT-qPCR. For the bioassay, no rectal swabs from pigs in the negative control (0 of 4) or the AHP disinfectant treatment groups (0 of 32) were positive for PEDV. Rectal swabs from all pigs within the positive control group (4 of 4) were positive for PEDV by RT-qPCR.
Under the conditions of this study, 1:16 and 1:32 dilutions of the AHP disinfectant successfully inactivated PEDV in swine feces on metal surfaces when applied at -10 °C with 40 or 60 min of contact time. This study also suggests that a positive RT-qPCR result for PEDV on an environmental sample should be expected when the AHP disinfectant is applied under freezing conditions, but does not necessarily indicate that an infectious dose of PEDV remains after disinfection.
The online version of this article (doi: 10.1186/s12917-017-1300-4) contains supplementary material, which is available to authorized users.
Url:
DOI: 10.1186/s12917-017-1300-4
PubMed: 29191202
PubMed Central: 5709983
Affiliations:
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Baker</name><affiliation wicri:level="2"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0004 1936 7312</institution-id><institution-id institution-id-type="GRID">grid.34421.30</institution-id><institution>Department of Veterinary Diagnostic and Production Animal Medicine,</institution><institution>Iowa State University College of Veterinary Medicine,</institution></institution-wrap>1809 S Riverside Dr, Ames, IA USA</nlm:aff><country>États-Unis</country><placeName><region type="state">Iowa</region></placeName><wicri:cityArea>1809 S Riverside Dr, Ames</wicri:cityArea></affiliation></author><author><name sortKey="Thomas, Paul R" sort="Thomas, Paul R" uniqKey="Thomas P" first="Paul R." last="Thomas">Paul R. 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Holtkamp</name><affiliation wicri:level="2"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="ISNI">0000 0004 1936 7312</institution-id><institution-id institution-id-type="GRID">grid.34421.30</institution-id><institution>Department of Veterinary Diagnostic and Production Animal Medicine,</institution><institution>Iowa State University College of Veterinary Medicine,</institution></institution-wrap>1809 S Riverside Dr, Ames, IA USA</nlm:aff><country>États-Unis</country><placeName><region type="state">Iowa</region></placeName><wicri:cityArea>1809 S Riverside Dr, Ames</wicri:cityArea></affiliation></author></analytic><series><title level="j">BMC Veterinary Research</title><idno type="eISSN">1746-6148</idno><imprint><date when="2017">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p id="Par1">Since its emergence in 2013, porcine epidemic diarrhea virus (PEDV) spread rapidly throughout the country due, in part, to contaminated livestock trailers. The objective of this study was to test the efficacy of an accelerated hydrogen peroxide (AHP) disinfectant for inactivating PEDV in swine feces on metal surfaces under freezing conditions. One 15.24 X 15.24 X 2.54 cm aluminum coupon, contaminated with swine feces, and randomly matched to one pig was the experimental unit. Eight treatment groups representing two AHP concentrations (1:16 and 1:32) in a 10% propylene glycol solution, two contact times in a -10 °C freezer (40 min and 60 min), and two levels of fecal contamination (5 mL and 10 mL) in addition to negative and positive control groups were evaluated. Forty 3-week-old pigs, intragastrically inoculated with the contents of the coupons after treatment, were used as a bioassay to determine the infectivity of PEDV after treatment. Infectivity was determined by detection of virus with a nucleocapsid (N) gene-based quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR) on rectal swabs collected from the inoculated pigs on days three and seven post-inoculation.</p></sec><sec><title>Results</title><p id="Par2">All post-treatment swabs from the negative control coupons were negative for PEDV via RT-qPCR. All post-treatment swabs collected from coupons in the AHP disinfectant treatment groups and the positive control group were positive for PEDV via RT-qPCR. For the bioassay, no rectal swabs from pigs in the negative control (0 of 4) or the AHP disinfectant treatment groups (0 of 32) were positive for PEDV. Rectal swabs from all pigs within the positive control group (4 of 4) were positive for PEDV by RT-qPCR.</p></sec><sec><title>Conclusions</title><p id="Par3">Under the conditions of this study, 1:16 and 1:32 dilutions of the AHP disinfectant successfully inactivated PEDV in swine feces on metal surfaces when applied at -10 °C with 40 or 60 min of contact time. This study also suggests that a positive RT-qPCR result for PEDV on an environmental sample should be expected when the AHP disinfectant is applied under freezing conditions, but does not necessarily indicate that an infectious dose of PEDV remains after disinfection.</p></sec><sec><title>Electronic supplementary material</title><p>The online version of this article (doi: 10.1186/s12917-017-1300-4) contains supplementary material, which is available to authorized users.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Pensaert, Mb" uniqKey="Pensaert M">MB Pensaert</name></author><author><name sortKey="De Bouck, P" uniqKey="De Bouck P">P de Bouck</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chasey, D" uniqKey="Chasey D">D Chasey</name></author><author><name sortKey="Cartwright, Sf" uniqKey="Cartwright S">SF Cartwright</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Stevenson, Gw" uniqKey="Stevenson G">GW Stevenson</name></author><author><name sortKey="Hoang, H" uniqKey="Hoang H">H Hoang</name></author><author><name sortKey="Schwartz, Kj" uniqKey="Schwartz K">KJ Schwartz</name></author><author><name sortKey="Burrough, Er" uniqKey="Burrough E">ER Burrough</name></author><author><name sortKey="Sun, D" uniqKey="Sun D">D Sun</name></author><author><name sortKey="Madson, D" uniqKey="Madson D">D Madson</name></author><author><name sortKey="Cooper, Vl" uniqKey="Cooper V">VL Cooper</name></author><author><name sortKey="Pillatzki, A" uniqKey="Pillatzki A">A Pillatzki</name></author><author><name sortKey="Gauger, P" uniqKey="Gauger P">P Gauger</name></author><author><name sortKey="Schmitt, Bj" uniqKey="Schmitt B">BJ Schmitt</name></author><author><name sortKey="Koster, Lg" uniqKey="Koster L">LG Koster</name></author><author><name sortKey="Killian, Ml" uniqKey="Killian M">ML Killian</name></author><author><name sortKey="Yoon, Kj" uniqKey="Yoon K">KJ Yoon</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pospischil, A" uniqKey="Pospischil A">A Pospischil</name></author><author><name sortKey="Stuedli, A" uniqKey="Stuedli A">A Stuedli</name></author><author><name sortKey="Kiupel, M" uniqKey="Kiupel M">M Kiupel</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lowe, J" uniqKey="Lowe J">J Lowe</name></author><author><name sortKey="Gauger, P" uniqKey="Gauger P">P Gauger</name></author><author><name sortKey="Harmon, K" uniqKey="Harmon K">K Harmon</name></author><author><name sortKey="Zhang, J" uniqKey="Zhang J">J Zhang</name></author><author><name sortKey="Connor, J" uniqKey="Connor J">J Connor</name></author><author><name sortKey="Yeske, P" uniqKey="Yeske P">P Yeske</name></author><author><name sortKey="Loula, T" uniqKey="Loula T">T Loula</name></author><author><name sortKey="Levis, I" uniqKey="Levis I">I Levis</name></author><author><name sortKey="Dufresne, L" uniqKey="Dufresne L">L Dufresne</name></author><author><name sortKey="Main, R" uniqKey="Main R">R Main</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dee, Sa" uniqKey="Dee S">SA Dee</name></author><author><name sortKey="Deen, J" uniqKey="Deen J">J Deen</name></author><author><name sortKey="Otake, S" uniqKey="Otake S">S Otake</name></author><author><name sortKey="Pijoan, C" uniqKey="Pijoan C">C Pijoan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dee, S" uniqKey="Dee S">S Dee</name></author><author><name sortKey="Deen, J" uniqKey="Deen J">J Deen</name></author><author><name sortKey="Burns, D" uniqKey="Burns D">D Burns</name></author><author><name sortKey="Douthit, G" uniqKey="Douthit G">G Douthit</name></author><author><name sortKey="Pijoan, C" uniqKey="Pijoan C">C Pijoan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dee, S" uniqKey="Dee S">S Dee</name></author><author><name sortKey="Torremorell, M" uniqKey="Torremorell M">M Torremorell</name></author><author><name sortKey="Thompson, B" uniqKey="Thompson B">B Thompson</name></author><author><name sortKey="Deen, J" uniqKey="Deen J">J Deen</name></author><author><name sortKey="Pijoan, C" uniqKey="Pijoan C">C Pijoan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dee, S" uniqKey="Dee S">S Dee</name></author><author><name sortKey="Burns, D" uniqKey="Burns D">D Burns</name></author><author><name sortKey="Douthit, G" uniqKey="Douthit G">G Douthit</name></author><author><name sortKey="Pijoan, C" uniqKey="Pijoan C">C Pijoan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Holtkamp, D" uniqKey="Holtkamp D">D Holtkamp</name></author><author><name sortKey="Thomas, P" uniqKey="Thomas P">P Thomas</name></author><author><name sortKey="Gerardy, K" uniqKey="Gerardy K">K Gerardy</name></author><author><name sortKey="Karriker, L" uniqKey="Karriker L">L Karriker</name></author><author><name sortKey="Ramirez, A" uniqKey="Ramirez A">A Ramirez</name></author><author><name sortKey="Zhang, J" uniqKey="Zhang J">J Zhang</name></author><author><name sortKey="Bates, J" uniqKey="Bates J">J Bates</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Thomas, P" uniqKey="Thomas P">P Thomas</name></author><author><name sortKey="Karriker, L" uniqKey="Karriker L">L Karriker</name></author><author><name sortKey="Ramirez, A" uniqKey="Ramirez A">A Ramirez</name></author><author><name sortKey="Zhang, J" uniqKey="Zhang J">J Zhang</name></author><author><name sortKey="Ellingson, J" uniqKey="Ellingson J">J Ellingson</name></author><author><name sortKey="Holtkamp, Dj" uniqKey="Holtkamp D">DJ Holtkamp</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Holtkamp, Dj" uniqKey="Holtkamp D">DJ Holtkamp</name></author><author><name sortKey="Myers, J" uniqKey="Myers J">J Myers</name></author><author><name sortKey="Thomas, Pr" uniqKey="Thomas P">PR Thomas</name></author><author><name sortKey="Karriker, La" uniqKey="Karriker L">LA Karriker</name></author><author><name sortKey="Ramirez, A" uniqKey="Ramirez A">A Ramirez</name></author><author><name sortKey="Zhang, J" uniqKey="Zhang J">J Zhang</name></author><author><name sortKey="Wang, C" uniqKey="Wang C">C Wang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Omidbakhsh, N" uniqKey="Omidbakhsh N">N Omidbakhsh</name></author><author><name sortKey="Sattar, S" uniqKey="Sattar S">S Sattar</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Omidbakhsh, N" uniqKey="Omidbakhsh N">N Omidbakhsh</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Madson, Dm" uniqKey="Madson D">DM Madson</name></author><author><name sortKey="Magstadt, Dr" uniqKey="Magstadt D">DR Magstadt</name></author><author><name sortKey="Arruda, Phe" uniqKey="Arruda P">PHE Arruda</name></author><author><name sortKey="Hoang, H" uniqKey="Hoang H">H Hoang</name></author><author><name sortKey="Sun, D" uniqKey="Sun D">D Sun</name></author><author><name sortKey="Bower, Lp" uniqKey="Bower L">LP Bower</name></author><author><name sortKey="Bhandari, M" uniqKey="Bhandari M">M Bhandari</name></author><author><name sortKey="Burrough, Er" uniqKey="Burrough E">ER Burrough</name></author><author><name sortKey="Gauger, Pc" uniqKey="Gauger P">PC Gauger</name></author><author><name sortKey="Pillatzki, Ae" uniqKey="Pillatzki A">AE Pillatzki</name></author><author><name sortKey="Stevenson, Gw" uniqKey="Stevenson G">GW Stevenson</name></author><author><name sortKey="Wilberts, Bl" uniqKey="Wilberts B">BL Wilberts</name></author><author><name sortKey="Brodie, J" uniqKey="Brodie J">J Brodie</name></author><author><name sortKey="Harmon, Km" uniqKey="Harmon K">KM Harmon</name></author><author><name sortKey="Wang, C" uniqKey="Wang C">C Wang</name></author><author><name sortKey="Main, Rg" uniqKey="Main R">RG Main</name></author><author><name sortKey="Zhang, J" uniqKey="Zhang J">J Zhang</name></author><author><name sortKey="Yoon, Kj" uniqKey="Yoon K">KJ Yoon</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Thrash, Rj" uniqKey="Thrash R">RJ Thrash</name></author><author><name sortKey="Robert, Rr" uniqKey="Robert R">RR Robert</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Davison, S" uniqKey="Davison S">S Davison</name></author><author><name sortKey="Benson, Ce" uniqKey="Benson C">CE Benson</name></author><author><name sortKey="Ziegler, Af" uniqKey="Ziegler A">AF Ziegler</name></author><author><name sortKey="Eckroade, Rj" uniqKey="Eckroade R">RJ Eckroade</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Block, Ss" uniqKey="Block S">SS Block</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bowman, As" uniqKey="Bowman A">AS Bowman</name></author><author><name sortKey="Nolting, Jm" uniqKey="Nolting J">JM Nolting</name></author><author><name sortKey="Nelson, Sw" uniqKey="Nelson S">SW Nelson</name></author><author><name sortKey="Bliss, N" uniqKey="Bliss N">N Bliss</name></author><author><name sortKey="Stull, Jw" uniqKey="Stull J">JW Stull</name></author><author><name sortKey="Wang, Q" uniqKey="Wang Q">Q Wang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Huss, Ar" uniqKey="Huss A">AR Huss</name></author><author><name sortKey="Schumacher, Ll" uniqKey="Schumacher L">LL Schumacher</name></author><author><name sortKey="Cochrane, Ra" uniqKey="Cochrane R">RA Cochrane</name></author><author><name sortKey="Poulsen, E" uniqKey="Poulsen E">E Poulsen</name></author><author><name sortKey="Bai, J" uniqKey="Bai J">J Bai</name></author><author><name sortKey="Woodworth, Jc" uniqKey="Woodworth J">JC Woodworth</name></author><author><name sortKey="Dritz, Ss" uniqKey="Dritz S">SS Dritz</name></author><author><name sortKey="Stark, Cr" uniqKey="Stark C">CR Stark</name></author><author><name sortKey="Jones, Ck" uniqKey="Jones C">CK Jones</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Greatorex, Js" uniqKey="Greatorex J">JS Greatorex</name></author><author><name sortKey="Digard, P" uniqKey="Digard P">P Digard</name></author><author><name sortKey="Curran, Md" uniqKey="Curran M">MD Curran</name></author><author><name sortKey="Moynihan, R" uniqKey="Moynihan R">R Moynihan</name></author><author><name sortKey="Wensley, H" uniqKey="Wensley H">H Wensley</name></author><author><name sortKey="Wreghitt, T" uniqKey="Wreghitt T">T Wreghitt</name></author><author><name sortKey="Varsani, H" uniqKey="Varsani H">H Varsani</name></author><author><name sortKey="Garcia, F" uniqKey="Garcia F">F Garcia</name></author><author><name sortKey="Enstone, J" uniqKey="Enstone J">J Enstone</name></author><author><name sortKey="Nguyen Van Tam, Js" uniqKey="Nguyen Van Tam J">JS Nguyen-Van-Tam</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Iowa</li></region></list><tree><country name="États-Unis"><region name="Iowa"><name sortKey="Baker, Kimberlee L" sort="Baker, Kimberlee L" uniqKey="Baker K" first="Kimberlee L." last="Baker">Kimberlee L. Baker</name></region><name sortKey="Holtkamp, Derald J" sort="Holtkamp, Derald J" uniqKey="Holtkamp D" first="Derald J." last="Holtkamp">Derald J. Holtkamp</name><name sortKey="Karriker, Locke A" sort="Karriker, Locke A" uniqKey="Karriker L" first="Locke A." last="Karriker">Locke A. Karriker</name><name sortKey="Karriker, Locke A" sort="Karriker, Locke A" uniqKey="Karriker L" first="Locke A." last="Karriker">Locke A. Karriker</name><name sortKey="Ramirez, Alejandro" sort="Ramirez, Alejandro" uniqKey="Ramirez A" first="Alejandro" last="Ramirez">Alejandro Ramirez</name><name sortKey="Ramirez, Alejandro" sort="Ramirez, Alejandro" uniqKey="Ramirez A" first="Alejandro" last="Ramirez">Alejandro Ramirez</name><name sortKey="Thomas, Paul R" sort="Thomas, Paul R" uniqKey="Thomas P" first="Paul R." last="Thomas">Paul R. Thomas</name><name sortKey="Thomas, Paul R" sort="Thomas, Paul R" uniqKey="Thomas P" first="Paul R." last="Thomas">Paul R. Thomas</name><name sortKey="Wang, Chong" sort="Wang, Chong" uniqKey="Wang C" first="Chong" last="Wang">Chong Wang</name><name sortKey="Zhang, Jianqiang" sort="Zhang, Jianqiang" uniqKey="Zhang J" first="Jianqiang" last="Zhang">Jianqiang Zhang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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